1 Folasaga
O le mamafa tele ma le tino puta ua avea ma fa'afitauli o le soifua maloloina i le lalolagi atoa ma e matua feso'ota'i ma fa'ama'i fatu fatu, toto maualuga, ma fa'afitauli o le tino. O vaila'au o lo'o i ai nei hypolipidemic ma hypoglycemic e masani ona fa'aalia ai a'afiaga, lea e fa'aosofia ai le fiafia i le saogalemu, mea fa'alenatura mo le fa'atonutonuina o le kulukose o le toto ma lipids.
Limu, fuala'au 'aina, ma fuala'au 'aina e mauoa i mea gaio'i fa'atasi ai -inflammatory, lipid-fa'aititia, ma le hypoglycemic.Sargassum fusiformefa'aalia le antioxidant ma le mamafa-gaioiga fa'atonutonu; curcumin e mafai ona faʻaogaina le lipid metabolism; o le punicalagin o lo'o fa'aalia ai le anti-inflammatory and lipid-a'afiaga. O le su'esu'eina o le -glucosidase ma le pancreatic lipase inhibitors i totonu o nei mea fa'alenatura e maua ai se fa'avae mo le atina'eina o fua fa'alenatura fa'alenatura hypoglycemic ma hypolipidemic.
2 Mea ma Metotia
2.1 Mea ma Reagents
Sargassum fusiforme, curcumin, punicalagin, -glucosidase, porcine pancreatic lipase, PNPG, 4-MUO, ma inhibitors masani (acarbose ma orlistat) sa fa'aogaina i lenei su'esu'ega.
2.2 Meafaigaluega
Sa fa'aogaina se masini fa'aaisa, fa'a'aisa fa'avili, centrifuge fa'a'aisa, fa'amu -te vevela tumau, su'esu'e microplate, ma fa'aoga vai mama.
2.3 Metotia Fa'ata'ita'i
2.3.1 Sauniuniga o le Sargassum fusiforme Aqueous Extract
Ole limu fa'asusu na maua mai ile fua ole 1:50, fa'avevela ile 85 tikeri mo le 6 itula, fa'a'a'ai ma fa'a'aisa-fa'amago ina ia maua ai le vai.
2.3.2 -Glucosidase Inhibition Su'ega
O le PNPG colorimetric method na faʻaaogaina e fuafua ai gaioiga faʻalavelave.
2.3.3 Su'esu'ega Fa'alavelave Lipase Pancreatic
4-MUO na avea ma mea'ai, ma na fuaina le malosi o le fluorescence e fa'atatau ai le fa'alavelave.
2.3.4 Fa'ata'ita'iga -Ta'atasi
Faʻavae i luga ole IC₅₀ faʻatusatusaga o gaioiga e tolu (150: 6: 2), na filifilia ai le tele o faʻasalalauga. I le 0.5 mg/mL le aofa'i atoa, o fua fa'atatau e fa'asaga i enzymes e lua na fuafuaina mo fua ta'itasi.
2.3.5 Fuafuaga Fa'afanua Tali
E tolu-tulaga, tolu-tulaga RSM mamanu na iloilo ai aafiaga o fua faatatau o mea e tolu i le fa'asaina o le enzyme e iloa ai le fua fa'atatau sili ona lelei.
3 I'uga ma Iloiloga
3.1 Fa'atusatusaga o IC₅₀ Tau
Na faʻaalia e Punicalagin le gaioiga sili ona malosi faʻalavelave:
-glucosidase:IC₅₀=0.420 mg/mL
Pancreatic lipase:IC₅₀=0.090 mg/mL
Curcumin na faʻaalia le gaioioiga feololo, aeSargassum fusiformeo lo'o fa'aalia ai le fa'aletonu vaivai (IC₅₀=38.22 mg/mL ma le 1.02 mg/mL).
Ole fua faatatau ole IC₅₀ e tusa150:6:2.
3.2 Fa'ata'ita'iga-Ta'atasi
3.2.1 Fa'atatau o le Sargassum fusiforme Extract
O le fa'asaina o le -glucosidase na fa'aitiitia i le maualuga o vaega, a'o le pancreatic lipase na fa'atuputeleina i le fua faatatau o le 450.450.
3.2.2 Fa'atatau o Curcumin
O le taofiofia o le -glucosidase na maualuga i le fua faatatau o le 4, ma le pancreatic lipase inhibition na maualuga i le 6. O le fua faatatau curcumin sili ona lelei na fuafuaina o le4.
3.2.3 Fa'atatau o Punicalagin
I lalo o tulaga tumau (450 Sargassum: 4 curcumin), faʻatupuina malie le punicalagin faʻatagaina le faʻamaoniaina o se fua faatatau sili ona lelei.2, fa'avaeina mo su'esu'ega RSM.
3.3 Su'esu'ega i luga ole Tali ma Fa'atonuga
Na faʻaalia e le RSM le aʻafiaga o mea e tolu i luga o le enzyme inhibition faʻavasegaina e pei ona taua i lalo:
Mo -glucosidase:
curcumin > punicalagin > Sargassum extract
Mo lipase pancreatic:
curcumin > Sargassum extract > punicalagin
O le fa'ata'ita'iga fa'ata'ita'iga na maua ai se fua fa'atatau o le tele o:
150:6:3 (Sargassum extract : curcumin : punicalagin)
Ole fua ole fa'ai'uga ole:
-glucosidase:47.77%
Pancreatic lipase:74.18%
O tau e fetaui lelei ma faʻataʻitaʻiga faʻataʻitaʻiga, faʻamaonia le faʻamaoni faʻataʻitaʻiga.
3.4 Enzyme Taofi ole Fua Fa'atatau
O le fua fa'atatau tu'ufa'atasi lelei na fa'aalia IC₅₀ tau o:
0.56 mg/mLmo -glucosidase
0.210 mg/mLmo lipase pancreatic
O nei mea na matua sili atu le malosi nai lo latouSargassum fusiformena'o ia, e fa'ailoa mai ai se fa'alelei fa'atasi pe a mae'a fa'atasi.
4 Talanoaga
O mea aoga ta'itasi e tolu o lo'o iai a'afiaga o le hypoglycemic ma le hypolipidemic, ma o le fua fa'atatau e fa'aalia ai le gaioiga fa'atasi. Ole fua faanatura sili ona lelei e saogalemu ma fa'aalia ai le malosi malosi mo le atina'eina o mea'ai aoga e fa'atatau ile toto kulukose ma le lipid tulafono faatonutonu. Ae ui i lea, e sili atui vivosu'esu'ega ma le usita'ia o mana'oga fa'atonutonu e mana'omia mo le atina'eina o oloa i le lumana'i.
